Alachlor Separation on Obelisc R Column
https://sielc.com/Application-Alachlor-Separation-on-Obelisc-and-Primesep-HPLC-ColumnsChromatogram
Description
Alachlor is a herbicide which inhibits the gibberellin pathway. Use of alachlor is banned by the European Union and the U.S. EPA. The EURL (European Union Reference Laboratory) includes alachlor in a pesticide analysis of teas and chamomile by liquid and gas chromatography. A single multiresidue method was developed using both Primesep B2 and Obelisc R analyze many pesticides. Obelisc R contains embedded ionic and hydrophobic groups which can assist in fine tuning separations, while Primesep B2 contains embedded basic ion-pairing groups. Method is LC/MS compatible and can be used as a general approach for analyzing alachlor.
Method Parameters
| Mobile Phase | Gradient MeCN – 10-70%, 15 min, 7 min hold |
|---|---|
| Buffer | Gradient AmAc pH 4,5- 20-50 mM, 15 min, 7 min hold |
| Flow Rate | 0.4 ml/min |
| Detection | UV, 250 nm |
| Class of Compounds | Insecticide, Herbicide, Fungicide, Hydrophobic, Ionizable |
| Analyzing Compounds | Alachlor |
HPLC Column Used
Obelisc R, 2.1×150 mm, 5 µm, 100A