4-CPA Analysis on Obelisc and Primesep Mixed-Mode Columns
https://sielc.com/Application-4-CPA-Analysis-on-Obelisc-and-Primesep-Mixed-Mode-ColumnsChromatogram
Description
4-CPA (4-Chlorophenoxyacetic acid) is a synthetic pesticide that mimics plant hormones called auxins. A single residue method for the EURL (European Union Reference Laboratory) was developed for 4-CPA and other acidic compounds using QuEChERS (Quick, Easy, Cheap, Effective, Rugged, and Safe) methodology. Retention of 4-CPA was controlled on both Obelisc R and a 3 micron Primesep 100 column of the same dimension. By using a gradient for the organic and ionic components of the mobile phase, the method is capable of separating dozens of pesticides quickly while remaining LC/MS compatible.
Method Parameters
| Mobile Phase | Gradient MeCN – 10-70%, 15 min, 7 min hold |
|---|---|
| Buffer | Gradient AmAc pH 3.0- 20-60 mM, 15 min, 7 min hold |
| Flow Rate | 0.4 ml/min |
| Detection | UV, 270 nm |
| Class of Compounds | Insecticide, Herbicide, Fungicide, Hydrophobic, Ionizable |
| Analyzing Compounds | 4-CPA |
HPLC Column Used
Primesep 100, 2.1×150 mm, 5 µm, 100A