2,4-D Retention on Primesep 100 and Obelisc R
https://sielc.com/application-2-4-d-retention-on-primesep-100-and-obelisc-rChromatogram
Description
2,4-D makes up half of the formula for Agent Orange, the defoliant that was used during the Vietnam War and Malayan Emergency. Commonly used in lawn and turf pesticides, 2,4-D is typically mixed with other pesticides such as mecoprop and dicamba. It was analyzed on Primesep 100 and Obelisc R. Primesep 100 separates using reverse-phase and acidic ion-pairing groups. Obelisc R has many modes of separation which allow it to tune retention. Method is LC/MS compatible and conditions could be replicated to retain many pesticides.
Method Parameters
| Mobile Phase | Gradient MeCN – 10-70% |
|---|---|
| Buffer | Gradient AmAc pH 3.0- 20-60 mM |
| Flow Rate | 0.4 ml/min |
| Detection | UV, 270 nm |
| Class of Compounds | Insecticide, Pesticide, Hydrophobic, Ionizable |
| Analyzing Compounds | 2,4-D |
HPLC Column Used
Primesep 100, 2.1×150 mm, 5 µm, 100A