Separation of Toluidine and Aminobiphenyl Isomers

HPLC-Applikation • Datum: 2015/07/06
Separation of Toluidine and Aminobiphenyl Isomers Chromatogramm

Toluidine isomers and aminobyphenyl isomers are organic compounds each with a substituted amine group. Primesep 200 was used to separate these isomers. Primesep 200 separates isomers by interacting with polar molecules, retaining basic compounds, and additionally by reverse phase mechanism.

Methodenparameter

ColumnPrimesep 200, 4.6×150 mm, 5 µm, 100 Å
Mobile PhaseMeCN/H2O
BufferAmFmPh3.5
Flow Rate1.0 mL/min
DetectionUV, 250 nm