Separation of Nucleic Bases
Primesep 200 separates with baseline resolution nucleic bases (uracil, thymine, cytosine, guanine, and adenine) by a combination of cation exchange and reversed phase. Uracil typically does not retain on reversed-phase column and is often used as an unretained void volume marker for C18 and C8 columns. Primesep 200 has an embedded anionic functional group which helps retain polar compounds polar and ion-exchange mechanisms. Excellent peak shape results with a mass spec compatible mobile phase of water, acetonitrile (MeCN, ACN) and trifluoracetic acid (TFA) with UV detection at 270 nm.
Methodenparameter
| Column | Primesep 200, 4.6*250 mm, 5 µm, 100 Å |
|---|---|
| Mobile Phase | MeCN/H2O – 10/90% |
| Buffer | TFA – 0.2% |
| Flow Rate | 0.5 mL/min, 1.0 mL/min |
| Detection | UV, 270 nm |