Separation of Model Compounds in Reversed-Phase and Mixed-Mode

Separation type: Liquid Chromatography Mixed-mode

High Performance Liquid Chromatography (HPLC) Method for Analysis of Adenosine , 3,4-Difluoroaniline , 4-Amino-2-Chloropyridine , 4-Amino-3-Chloropyridine , 2-Amino-5-Methylthiadiazole , 2-Amino-5-methyl-thiazole , 4-Ethylaniline , 5-Aminoindole .

Many compounds are difficult, if not impossible, to separate on reverse-phase columns in HPLC. Other compounds cannot be separated on ion-exchange columns. That’s where the mixed-mode columns come in. By using a stationary phase with both hydrophobic and ion-exchange properties, allows the chromatographer to have additional controls over separation conditions. Here, we demonstrate the separation of compounds that can’t be achieved on a C18 column. By using both an organic gradient and buffer gradient of ammonium formate (AmFm), we can separate structurally similar compounds that can’t be separated on a reverse-phase column alone.