Reverse Elution Order in Separation of Alkali Cation

HPLC-Applikation • Datum: 2006/08/12
Reverse Elution Order in Separation of Alkali Cation Chromatogramm

Primesep C separates a mixture of inorganic cations, potassium, sodium, and lithium, by a combination of cation exchange and complex formation. The peak order is reversed on the Primesep C column compared to the order normally obtained on columns that rely on cation exchange alone. The mobile phase mixture of water, acetonitrile (MeCN, ACN) and ammonium acetate is compatible with UV and evaporative light scattering detection (ELSD).

Methodenparameter

ColumnPrimesep C, 4.6×50 mm, 5 µm, 100 Å
Mobile PhaseMeCN/H2O
BufferAmAc
Flow Rate1.0 mL/min
DetectionELSD50C