HPLC Separation of Ten Cannabinoids on Cannsep A Column

High Performance Liquid Chromatography (HPLC) Method for Analysis of Cannabidivarin (CBDV) , Cannabidiol (CBD) , Cannabigerol (CBG) , Cannabinol (CBN) , Tetrahydrocannabinol (THC) , Delta-9-Tetrahydrocannabinol (THC) , Tetrahydrocannabinolic acid (THCA-A) , Tetrahydrocannabivarin (THCV) , Cannabidiolic acid (CBDA) , Delta-8-Tetrahydrocannabinol (8-THC) .

Cannabidivarin ( CBDV ) is a non-intoxicating psychoactive cannabinoid with the chemical formula C19H26O2 . Due to it demonstrating anticonvulsive properties, it is actively in Phase-2 trials for adult epilepsy, childhood epilepsy, and for Prader-Willi Syndrome.

Tetrahydrocannabivarin ( THCV , THV ) is a cannabinoid receptor type 1 antagonist with the chemical formula C19H26O2 . It is prevalent in certain strains of Cannabis in central Asia and southern Africa. It is currently being researched as a potential treatment for obesity-associated glucose intolerances. It is also said to reduce appetite and have energizing effects.

Cannabidiol ( CBD ) is a phytocannabinoid with the chemical formula C21H30O2 . It is used in a variety of ways. It’s only confirmed medical use is as epilepsy medication. It may help with pain, sleep, and addiction as an alternative to opioids, but due to limited clinical evidence, it’s regulations are dependent on the state. It is advised against using it during pregnancy due to unknown effects on fetal and infant development.

Cannabidiolic Acid ( CBDA ) is a precursor to Cannabidiol with the chemical formula C22H30O4 . Research suggests that it might have anti-inflammatory, anti-emetic, anti-convulsant, and anti-carcinogenic properties, but not enough research has been done into it for it to be used as widely as other cannabinoids and the like.

Cannabigerol ( CBG ) is a non-psychoactive cannabinoid with the chemical formula C21H32O2 . It is a minor constituent of cannabis. CBG that is derrived from marijuana is illegal, while CBG derived from hemp is legal as long as the THC content of hemp is less than 0.3% of dry weight. It was sold illegally by certain companies under unproven claims of being treatment against the COVID-19 virus and inflammation.