HPLC Separation Of Mixture of Pyridylacetic Acids And Their Derivatives
High Performance Liquid Chromatography (HPLC) Method for Analysis of 2-Pyridylacetic acid hydrochloride , 3-Pyridylacetic acid hydrochloride , 4-Pyridylacetic acid hydrochloride , Nicotinic Acid/Niacin (3-pyridinecarboxylic acid) , 3-Acetylpyridine , Pyridyl-3-thioacetomorpholide
Pyridylacetic Acids are a class of compounds with a pyridine ring and an acetic acid. These acids are typically building blocks found in organic synthesis/ On occasion, they can also act as human xenobiotic metabolites.
Nicotinic acid , also known as niacin and vitamin B3, is a water-soluble B vitamin with C6H5NO2molecular formula. It is crucial for energy production.
Derrivatives are compounds formed when the parent compound goes through a chemical recation, replacing atoms with different ones, leading to different physical and chemical properties.
2-Pyridylacetic acid hydrochloride , 3-Pyridylacetic acid hydrochloride , 4-Pyridylacetic acid hydrochloride , Nicotinic Acid/Niacin (3-pyridinecarboxylic acid) , 3-Acetylpyridine , Pyridyl-3-thioacetomorpholide can be retained and analyzed using the Primesep 200 stationary phase column. The analysis utilizes a gradient method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a formic acid buffer. Detection is performed using UV.
Methodenparameter
| Column | Primesep 200, 4.6 x 250 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN/H2O Gradient – 5-50% |
| Buffer | CH2O2 – 0.02-0.05% |
| Flow Rate | 1.0ml/min |
| Detection | UV, 270 nm |