HPLC Separation of Glucose 6- Phosphate and Glucose 1- Phosphate on Newcrom B Column
High Performance Liquid Chromatography (HPLC) Method for Analysis of Glucose 6-Phosphate , Glucose 1- Phosphate .
Structural isomer separation poses a challenging task when using reverse-phase chromatography. However both glucose variants, which only differ to which carbon the phosphate group is attached to, can be separated in HPLC isocratically on a reverse-phase mixed-mode anion-exchange Newcrom B column. What differentiates, the Newcrom B phase from other silica-based Analysis of glucose phosphate is important because glucose-6-phosphate is the product of the first step in glycolysis metabolic pathway and glucose-1-phosphate is the first step in glycogenolysis. Glucose-6-phosphate is converted to glucose-1-phosphate and vice versa by the phosphoglucomutase enzyme in biological systems.
Methodenparameter
| Column | Newcrom B, 4.6 x 150 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN/H2O – 50/50% |
| Buffer | Formic Acid – 0.5% |
| Flow Rate | 1.0 mL/min |
| Detection | ELSD, 40 °C MS- compatible mobile phase |