HPLC – MS Method for Analysis of Rifampicin and Oxidation product on Primesep 100 Column
Rifampicin is an important antibiotic used primarily in the treatment of tuberculosis. Let’s delve into both rifampicin and its metabolite, rifampicin quinone:
Uses: It’s mainly used for the treatment of tuberculosis, often in combination with other antitubercular agents. It can also be used to treat leprosy, and as prophylaxis for Neisseria meningitidis (meningococcal) infections.
Rifampicin can be retained, separated and analyzed on a Primesep 100 mixed-mode stationary phase column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and a ammonium format as a buffer. This analysis method can be detected using UV at 270 nm, an Evaporative Light Scattering Detector (ELSD), or any other evaporative detection method (CAD, ESI-MS)
Methodenparameter
| Column | Primesep 100, 2.1 x 100 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN – 70%, |
| Buffer | Ammonium Formate pH 3.0-20 mM |
| Flow Rate | 0.2 mL/min |
| Detection | UV, 270 nm, SIM823 +, SIM821 + |