HPLC – MS Method for Analysis of Nimesulide on Newcrom R1 Column
Nimesulide is a non-steroidal anti-inflammatory drug (NSAID) that possesses analgesic and antipyretic properties. Below is some information about Nimesulide:
Nimesulide can be retained, separated and analyzed on a Newcrom R1 reverse phase stationary phase column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and a ammonium format as a buffer. This analysis method can be detected using UV at 300 nm, an Evaporative Light Scattering Detector (ELSD), or any other evaporative detection method (CAD, ESI-MS)
Methodenparameter
| Column | Newcrom R1, 2.1 x 100 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN – 50%, |
| Buffer | Ammonium Formate pH 3.0-20 mM |
| Flow Rate | 0.2 mL/min |
| Detection | UV 300 nm, SIM307.2- |