HPLC – MS Method for Analysis of Diquat on Obelisc R Column

HPLC-Applikation • Datum: 2023/12/22
HPLC – MS Method for Analysis of Diquat on Obelisc R Column Chromatogramm

Diquat is a non-selective contact herbicide used in agriculture and landscaping. It’s known for its effectiveness in controlling a wide range of weeds and plants.

diquat is a potent herbicide used for controlling a broad spectrum of weeds and as a desiccant in agriculture. While effective, its toxicity and environmental impact necessitate careful handling and adherence to regulatory guidelines.

Diquat can be retained and analyzed on a Obelisc R mixed-mode stationary phase column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and a ammonium format as a buffer. This analysis method can be detected an Evaporative Light Scattering Detector (ELSD), or any other evaporative detection method (CAD, ESI-MS)

Methodenparameter

ColumnObelisc R, 2.1 x 100 mm, 5 µm, 100 Å, dual ended
Mobile PhaseMeCN – 50%,
BufferAmmonium Formate pH 3.0-50 mM
Flow Rate0.2 mL/min
DetectionSIM183 +, UV 310 nm