HPLC MS Method for Analysis of Alexidine on Primesep N Column
Separation type: Liquid Chromatography Reversed-phase
Alexidine is a chemical compound that belongs to the class of bis-biguanide antiseptics. It is known for its antimicrobial properties and is used in various products for disinfection and antiseptic purposes. Alexidine has broad-spectrum antimicrobial activity, and it is commonly used in healthcare settings for its effectiveness against bacteria, fungi, and some viruses.
Alexidine can be retained and analyzed on a Primesep N mixed-mode stationary phase column using an analytical method with a simple mobile phase of water, Acetonitrile (MeCN) , and a ammonium formate as a buffer. This analysis method can be detected using LC MS.
Methodenparameter
| Column | Primesep N, 2.1 x 100 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN/H2O – 85/15% |
| Buffer | Ammonium Formate pH 3.0 – 10 mM |
| Flow Rate | 0.2 mL/min |
| Detection | SIM255+, 509+ |