HPLC Method of Ofloxacin in Ofloxacin 0.3 % Ear Drops on Primesep D Column
High Performance Liquid Chromatography (HPLC) Method for Analysis of Ofloxacin
Ofloxacin is a common antibiotic with the chemical formula C18H20FN3O4 . It is typically taken by mouth or injected into a vein to treat variety of bacterial infections. Those infections include pneumonia, prostatitis, and the plague. As eye and ear drops, it is also used to treat superficial bacterial infections of the eye and otitis media. As a fluoroquinolone, it is well tolerated, but may cause nausea, vomiting, and diarrhea as a side effect.
Ofloxacin can be measured at low UV. Using a Primesep D reverse-phase column and a mobile phase consisting of water and an acetonitrile (MeCN) gradient with a sulfuric acid (H2SO4) buffer, Ofloxacin can be retained. This analysis method can be UV detected at 253 nm.
Methodenparameter
| Column | Primesep D, 4.6 x 150 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | Gradient MeCN – 10-20%, 10 min |
| Buffer | H2SO4 – 0.1% |
| Flow Rate | 1.0 mL/min |
| Detection | UV 253 nm |