HPLC Method for Separation of S-Nitroso-L-glutathione and Glutathione oxidized on Primesep 200 Column
Separation type: Liquid Chromatography Mixed-mode
Both S-Nitroso-L-glutathione (GSNO) and oxidized glutathione (GSSG) are forms of glutathione, a tripeptide consisting of the amino acids glutamic acid, cysteine, and glycine. Glutathione plays a significant role in maintaining the redox (reduction-oxidation) balance in cells, but GSNO and GSSG each have unique characteristics:
Each form of glutathione plays a unique role in cell signaling and defense mechanisms. While they are related, the specific effects of GSNO and GSSG within cells can be quite different due to their distinct chemical structures and reactivities.
These compounds can be retained, separated, and analyzed using a reverse-phase Primesep 200, 3.2 x 100 mm, 5 µm, 100 Å, dual ended column. The mobile phase for this method consists of water, acetonitrile (MeCN), and Sulfuric acid, which serves as a buffer. This analytical method can be
Methodenparameter
| Column | Primesep 200, 3.2 x 100 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN -10% |
| Buffer | H2SO40.2% |
| Flow Rate | 1.0 mL/min |
| Detection | UV 200, 355 nm |