HPLC Method for Lorazepam Injection on Newcrom R1 Column
High Performance Liquid Chromatography (HPLC) Method for Analysis of Lorazepam.
Lorazepam is a short-acting benzodiazepine medication commonly used as a sedative and anxiolytic of choice in the inpatient setting owing to its fast onset of action when administered intravenously. Lorazepam is one of the few sedative-hypnotics with a relatively clean side effect profile. It has the chemical formula C15H10Cl2N2O2 .
You can find detailed UV spectra of Lorazepam , Benzyl alcohol and information about its various lambda maxima by visiting the following link.
Lorazepam can be retained in HPLC on Newcrom R1 reverse-phase column with the simple isocratic mobile phase consisting of acetonitrile (MeCN) and water. The analysis method can be UV detected at 275 nm.
Methodenparameter
| Column | Newcrom R1, 4.6 x 150 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN – 35% |
| Buffer | No |
| Flow Rate | 1.0 mL/min |
| Detection | UV 275 nm |