HPLC Method for Analysis of Tribromoethanol (TBE) on Primesep B Column
Tribromoethanol (TBE) is a chemical compound with the molecular formula C2H3Br3O . It is a halogenated alcohol commonly used as an anesthetic agent in laboratory animals, particularly rodents.
Tribromoethanol (TBE) can be retained, and analyzed using a Primesep B mix mode stationary phase column. The analysis utilizes a isocratic method with a simple mobile phase consisting of water, acetonitrile (MeCN), and phosphoric acid as a buffer. Detection is carried out using UV.
Methodenparameter
| Column | Primesep B, 4.6 x 150 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN/H2O – 30/70% |
| Buffer | H3PO4 – 0.1% |
| Flow Rate | 1.0 mL/min |
| Detection | UV 230 nm |