HPLC Method for Analysis of Thiourea on Primesep P Column
Separation type: Liquid Chromatography Mixed-mode
Thiourea is a versatile chemical compound with the formula (NH2)2CS. It is structurally similar to urea, except that the oxygen atom is replaced by a sulfur atom. It is a white crystalline solid when pure.
Thiourea is used in a variety of applications. It is a reagent in organic synthesis, often used in the toning of silver-gelatin photography prints, as a component of hair preparations and bleaches, and as a radioprotective agent in cancer therapy.
In agriculture, thiourea is used as a soil treatment agent to promote germination and stimulate flowering and fruiting.
Using a Primesep P Mixed-mode phase column and a mobile phase consisting of water and Acetonitrile (MeCN) with no buffer, thiourea can be retained and analyzed. This analysis method can be UV detected at 200 nm.
LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.
Methodenparameter
| Column | Primesep P, 4.6 x 250 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN/H2O – 5/95% |
| Buffer | None |
| Flow Rate | 1.0 mL/min |
| Detection | UV 200 nm |
| Limit of Detection | UV 1 ppb |
| Injection Volume | 1 µl |