HPLC Method For Analysis Of Tetracycline on Primesep 100 Column
High Performance Liquid Chromatography (HPLC) Method for Analysis of Tetracycline
Tetracycline is a broad spectrum antibiotic, that is, it is active against many different types of bacteria. It is effective against Chlamydia psittaci, Neisseria gonorrhoeae, Haemophilus influenzae, Streptococcus pneumoniae, Mycoplasma pneumoniae, Chlamydia trachomatis, and others. Tetracycline prevents the growth of bacteria by preventing the bacteria from manufacturing proteins they need to survive.
Tetracycline can be retained on the Primesep 100 mixed-mode column using an isocratic analytical method with a simple mobile phase of water, acetonitrile (MeCN, ACN), and sulphuric acid (H2SO4) buffer. The analysis method can be UV detected at 200 nm.
Methodenparameter
| Column | Primesep 100, 4.6 x 150 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN/H2O – 50/50% |
| Buffer | H2SO4 – 0.5% |
| Flow Rate | 1.0 mL/min |
| Detection | UV, 200 nm |