HPLC Method For Analysis Of Sulfamethoxazole and Trimethoprim on Primesep 100 Column
Separation type: Liquid Chromatography Mixed-mode
High Performance Liquid Chromatography (HPLC) Method for Analysis of Sulfamethoxazole and Trimethoprim
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A , AH , B , and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
Methodenparameter
| Column | Primesep 100, Newcrom R1 4.6×150 mm, 5 µm, 100 Å |
|---|---|
| Mobile Phase | MeCN/H2O – 40/60% |
| Buffer | H2SO4 – 0.4% |
| Flow Rate | 1.0 mL/min |
| Detection | UV, 200 nm, |