HPLC Method for Analysis of Strychnine
Strychnine are colorless to white crystals used as a rodenticide and pesticide . It can kill small vertebrates such as birds and rodents. The chemical is toxic if swallowed, or absorbed through the eyes or mouth . It also produces toxic oxides of nitrogen if combusted. Primesep 200, a reverse phase column , contains embedded acidic ionizable groups and can retain Strychnine. The method is UV compatible and can be used as a general approach for analyzing similar compounds.
Methodenparameter
| Column | Primesep 200, 3.2×100 mm, 5 µm, 100 Å |
|---|---|
| Mobile Phase | Gradient MeCN – 5-50% |
| Buffer | Gradient H3PO4 – 0.05-0.3%, 5 min, 4 min hold |
| Flow Rate | 0.5 mL/min |
| Detection | UV, 270 nm |