HPLC Method for Analysis of Simazine on Primesep 100 Column
Separation type: Liquid Chromatography Mixed-mode SIELC Technologies
Simazine is a herbicide that belongs to the class of triazine herbicides . It is commonly used for controlling broadleaf weeds and grasses in agricultural and non-agricultural settings.
Simazine can be retained, separated and analyzed using a Primesep 100 mixed-mode stationary phase column. The analysis employs a gradient method with a simple mobile phase comprising water, acetonitrile (MeCN), and sulfuric acid as a buffer. This method allows for detection using UV 250 nm.
You can find detailed UV spectra of Simazine and information about its various lambda maxima by visiting the following link .
Methodenparameter
| Column | Primesep 100, 4.6 x 50 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN – 50% |
| Buffer | H2SO4 – 0.1% |
| Flow Rate | 1.0 mL/min |
| Detection | UV 250 nm |
| Limit of Detection | 30 ppb |