HPLC Method for Analysis of Pyridine on Primesep 100 Column
Separation type: Liquid Chromatography Mixed-mode SIELC Technologies
Pyridine is an aromatic heterocyclic organic compound with the chemical formula C5H5N. It is structurally related to benzene, with one methine group (=CH−) replaced by a nitrogen atom.
Pyridine can be retained, separated and analyzed using a Primesep 100 mixed-mode stationary phase column. The analysis employs a gradient method with a simple mobile phase comprising water, acetonitrile (MeCN), and sulfuric acid as a buffer. This method allows for detection using UV 250 nm.
You can find detailed UV spectra of Pyridine and information about its various lambda maxima by visiting the following link .
Methodenparameter
| Column | Primesep 100, 4.6 x 150 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN – 70% |
| Buffer | H2SO4 – 0.05% |
| Flow Rate | 1.0 mL/min |
| Detection | UV 250 nm |
| Limit of Detection | 5 ppb |