HPLC Method for Analysis of Pentosidine
High Performance Liquid Chromatography (HPLC) Method for Analysis of Pentosidine
Pentosidine is a advanced glycation end-product (AGE) with C17H26N6O4 • CF3COOHchemical formula. Since it is a fluorescent biomarker, it is often used as an indicator in studies of aging and chronic diseases including diabetes and uremia.
Pentosidine can be retained and analyzed using the Obelisc R stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with an ammonium acetate buffer. Detection is performed using UV.
Methodenparameter
| Column | Obelisc R, 4.6 x 150 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN – 20% |
| Buffer | Ammonium acetate |
| Flow Rate | 1.0 mL/min |
| Detection | UV |