HPLC Method For Analysis Of Patulin on Newcrom R1 Column
High Performance Liquid Chromatography (HPLC) Method for Analysis of Patulin.
Patulin is a toxic chemical contaminant produced by several species of mold, especially within Byssochlamys Aspergillus and Penicillium. It is the most common mycotoxin found in apples and apple-derived products such as juice, cider, compotes and other food intended for young children.
Patulin can be retained in HPLC on Newcrom R1 reverse-phase column with the simple isocratic mobile phase consisting of acetonitrile (MeCN, ACN), water and Phosphoric Acid (H3PO4) buffer. The analysis method can be UV detected at 275 nm.
Methodenparameter
| Column | Newcrom R1, 3.2 x 100 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN – 10% |
| Buffer | H3PO4 – 0.2% |
| Flow Rate | 0.5 mL/min |
| Limit of Detection | 0.002 ppm |