HPLC Method for Analysis of Oligonucleotides 37 mer on BIST A Column
Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies
This is a single-stranded DNA molecule that is 37 nucleotides long, and its sequence is as follows:
GGC CAC GCG TCG ACT AGT ACT TTT TTT TTT TTT TTT TTT T
The sequence contains the nucleotides G, C, A, and T, which represent the four different bases in DNA. The sequence also contains a stretch of thymine (T) nucleotides at the end, which serves as a poly-T tail. This tail is often added to oligonucleotides used in PCR and other molecular biology techniques to increase their binding affinity to the target DNA sequence.
The sequence also contains a specific sequence that can be used for hybridization or amplification purposes. The sequence GGC CAC GCG TCG ACT AGT ACT is known as the “primer” sequence, and it can be used to specifically bind to a complementary DNA sequence in PCR or other molecular biology techniques.
Overall, the sequence shown is an example of a synthetic oligonucleotide that can be used in various molecular biology applications.
Methodenparameter
| Column | BIST A, 4.6 x 100 mm, 5 µm, 100 Å, surface coated |
|---|---|
| Mobile Phase | Gradient MeCN – 50-5%, 10 min |
| Buffer | Mg acetate pH 4.0 – 20 mM |
| Flow Rate | 1.0 mL/min |
| Detection | UV 260 nm |