HPLC Method for Analysis of N,N-Bis (2-hydroxy)-p-phenylenediamine on Primesep 100 Column
N,N-Bis(2-hydroxyethyl)-p-phenylenediamine (often abbreviated as N,N-Bis(2-hydroxyethyl)-PPD) is a chemical compound that is a derivative of p-phenylenediamine (PPD), a common ingredient in hair dyes.
The N,N-Bis(2-hydroxyethyl)-p-phenylenediamine be retained and analyzed using a Primesep 100 mixed-mode stationary phase column. The analysis employs an isocratic method with a simple mobile phase comprising water, acetonitrile (MeCN), and sulfuric acid as a buffer. This method allows for detection using UV at 210 nm
Methodenparameter
| Column | Primesep 100, 4.6 x 150 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN/H2O – 30/70% |
| Buffer | H2SO4 -0.2% |
| Flow Rate | 1.0 mL/min |
| Detection | UV 210 nm |
| Injection Volume | 3 µl |