HPLC Method for Analysis of Nitroglycerin on Newcrom R1 Column
High Performance Liquid Chromatography (HPLC) Method for Analysis of Nitroglycerin
Nitroglycerin is a chemical compound with C3H5N3O9as it’s molecular formula. Historically, Nitroglycerin has been used as an explosive. These days, however, it is more commonly used as a vasodilator medication through widening blood vessels.
Nitroglycerin can be retained and analyzed using the Newcrom R1 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a phosphoric acid buffer. Detection is performed using UV.
Methodenparameter
| Column | Newcrom R1, 4.6 x 100 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN/H2O – 50/50% |
| Buffer | H3PO4 – 0.1% |
| Flow Rate | 1.0 mL/min |
| Detection | UV, 215 nm |