HPLC Method for Analysis of Myelin Basic Protein (85-99) Peptide Antagonist on BIST B+ Column
High Performance Liquid Chromatography (HPLC) Method for Analysis of Myelin Basic Protein Peptide Antagonist
Myelin Basic Protein Peptide Antagonist is a synthetic peptide antagonist with the molecular formula C70H114N18O21 • XCF3COOH . When expressing an MBP (85-99), it inhibits the production of IL-2 in T cell hybridoma cells. MBP peptide antagonist also delays MBP (85-99)-induced experimental autoimmune encephalomyelitis and reduces symptom severity.
Myelin Basic Protein Peptide Antagonist can be retained and analyzed using the BIST B+ stationary phase column. The analysis utilizes a gradient method with a simple mobile phase consisting of water and acetonitrile (MeCN). Detection is performed using UV.
Methodenparameter
| Column | BIST B+, 4.6 x 50 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | Gradient MeCN – 70/30% – 50/50%, 10 min |
| Buffer | Sulfuric Acid |
| Flow Rate | 1.0 mL/min |
| Detection | UV 200 |