HPLC Method for Analysis of Methylprednisolone on Primesep B Column
Methylprednisolone is a synthetic corticosteroid with anti-inflammatory and immunosuppressive properties. It is a derivative of prednisolone and, like prednisone, belongs to the class of glucocorticoids. Methylprednisolone is used for various medical conditions to reduce inflammation and modify the immune response.
Methylprednisolone can be retained, and analyzed using a Primesep B mixed-mode stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water, acetonitrile (MeCN), and sulfuric acid as a buffer. Detection is achieved using UV 245 nm
Methodenparameter
| Column | Primesep B, 4.6 x 150 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN/H2O – 35% |
| Buffer | H2SO4 – 0.1% |
| Flow Rate | 1.0 mL/min |
| Detection | UV 245 nm |