HPLC Method for Analysis of Methionine on Primesep 100 Column
Separation type: Liquid Chromatography Mixed-mode SIELC Technologies
Methionine is an essential amino acid, meaning the body cannot synthesize it, so it must be obtained through the diet. It’s crucial for many bodily functions, including:
Protein Synthesis: Methionine is a building block for proteins.
Methylation: It acts as a precursor to S-adenosylmethionine (SAMe), which is involved in methylation processes that are vital for DNA regulation, detoxification, and neurotransmitter production.
Antioxidant Function: Methionine contributes to the synthesis of cysteine, which is a precursor to glutathione, one of the body’s most important antioxidants.
Liver Health: It’s also important for liver function and fat metabolism.
Methodenparameter
| Column | Primesep 100, 4.6 x 150 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN – 35% |
| Buffer | H2SO4 – 0.05% |
| Flow Rate | 1.0 mL/min |
| Detection | UV 200 nm |
| Limit of Detection | 40 ppb |