HPLC Method for Analysis of Meclizine on Primesep B Column
High Performance Liquid Chromatography (HPLC) Method for Analysis of Meclizine
Meclizine is a histamine H1 antagonist and a synthetic piperazine. It is used to prevent and treat motion sickness, vertigo, and nausea during pregnancy. The side effects that may occur include drowsiness, dry mouth, and tiredness. The method is UV compatible and can be used as a general approach for analyzing similar compounds.
Meclizine can be retained and analyzed using the Primesep B stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a phosphoric buffer. Detection is performed using UV.
Methodenparameter
| Column | Primesep B, 4.6 x 150 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN/H2O – 10/90% |
| Buffer | H3PO4 – 0.2% |
| Flow Rate | 1.0 mL/min |
| Detection | UV, 232 nm |