HPLC Method for Analysis of Iodide on Primesep B Column
Separation type: Liquid Chromatography Mixed-mode
Iodide is an ion with the symbol I – . There is currently a worldwide iodine deficiency, which is a leading preventable cause of intellectual disability, has caused some governments to mandate iodized salt to be sold. It is naturally occurring as a mineral, Iodargyrite.
Iodide can be retained and analyzed using the [mode] stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a perchloric acid buffer. Detection is performed using UV.
You can find detailed UV spectra of Iodide and information about its various lambda maxima by visiting the following link .
Methodenparameter
| Column | Primesep B, 3.2 x 100 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN/H2O – 50/50% |
| Buffer | H2SO4 – 0.5% |
| Flow Rate | 0.5 mL/min |
| Detection | UV 228 nm |