HPLC Method for Analysis of Glycine Methyl Ester Hydrochloride
High Performance Liquid Chromatography (HPLC) Method for Analysis of Glycine methyl ester , Glycine
Glycine Methyl Ester Hydrochloride is a water soluble organic compound that is typically used in synthesis of pharmaceuticals and pesticides. Besides that, it is also used as a flavor enhancer and nutritional supplement.
Glycine Methyl Ester Hydrochloride can be retained and analyzed using the Primesep-A stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a sulfuric acid buffer. Detection is performed using UV.
Methodenparameter
| Column | Primesep A, 3.2 x 150 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN/H2O – 10/90% |
| Buffer | H2SO4 – 0.05% |
| Flow Rate | 0.05 mL/min |
| Detection | UV, 200 nm |