HPLC Method for Analysis of Estetrol, Estiol, Estrone, and Besta-Estradiol Without Buffer on Chromni Column

HPLC-Applikation • Datum: 2025/09/16
HPLC Method for Analysis of Estetrol, Estiol, Estrone, and Besta-Estradiol Without Buffer on Chromni Column Chromatogramm

High Performance Liquid Chromatography (HPLC) Method for Analysis of Estrone , Estradiol , Estriol , Estetrol

Estetrol is an estrogenic steroid hormone compound with the molecular formula C18H24O4 .

Properties: Appearance: Typically a white, odorless, powder.

Molecular weight: ~270.4 g/mol

Solubility: Soluble in water.

Uses: Production of contraceptives.

Methodenparameter

ColumnChromni, 4.6 x 150 mm, 3 µm, 100 Å, dual ended
Mobile PhaseMeCN – 90%
BufferNone
Flow Rate1.0 mL/min
DetectionUV 280 nm