HPLC Method for Analysis of Econazole on Primesep 200 Column
Econazole is an antifungal medication primarily used to treat a variety of fungal skin infections. It belongs to the imidazole class of antifungal agents. Here are some key points about econazole:
Like other imidazole antifungals, econazole works by inhibiting the enzyme responsible for producing ergosterol, a key component of the fungal cell membrane. A lack of ergosterol disrupts the integrity of the fungal cell membrane, leading to the death of the fungus.
Econazole can be retained, and analyzed on a Primesep 200 mixed-mode stationary phase column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and a sulfuric acid as a buffer. This analysis method can be detected in the UV 200 nm.
Methodenparameter
| Column | Primesep 200, 4.6 x 150 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | Gradient MeCN – 40-70%, 10 min |
| Buffer | H2SO4 – 0.2% |
| Flow Rate | 1.0 mL/min |
| Detection | UV, 200 nm |