HPLC Method for Analysis of Cystine on Primesep 100 Column
High Performance Liquid Chromatography (HPLC) Method for Analysis of Cystine
Cystine is an organic compound with the molecular formula C6H12N2O4S2 .
Properties: Appearance: Typically appears yellowish with a waxy, compact, and partially opaque texture.
Molecular weight: ~240.3 g/mol
Solubility: Soluble in water with HCL or NaOH.
Uses: Plays a crucial role in various biological processes due to its ability to stabilize protein structures and its role as a precursor to the antioxidant glutathione.
Methodenparameter
| Column | Primesep 100, 4.6 x 150 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN – 20% |
| Buffer | H2SO4 – 0.2% |
| Flow Rate | 1.0 mL/min |
| Detection | UV 200 nm |