HPLC Method for Analysis of Cypermethrin on Primesep 200 Column
Separation type: Liquid Chromatography Mixed-mode
Cypermethrin is a synthetic pyrethroid with neurotoxic properties that is typically used as an insecticide. Cypermethrin can be retained, analyzed, and separated on a Primesep 200 column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and a Formic acid (FA) ionic modifier. This analysis method can be detected in the low UV regime at 270 nm as well as with an Evaporative Light Scattering Detector (ELSD) or any other evaporative detection method (CAD, ESI-MS).
Methodenparameter
| Column | Primesep 200, 4.6 x 150 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | Gradient MeCN – 60-80%, 10 min |
| Buffer | Formic Acid – 0.5% |
| Flow Rate | 1.0 mL/min |
| Detection | UV 270 nm, ELSD, 50C |