HPLC Method for Analysis of Crystal Violet on Primesep 100 Column
Separation type: Liquid Chromatography Mixed-mode
Crystal Violet, also known as Methyl Violet 10B, is a synthetic dye belonging to the triarylmethane dye family. It has the molecular formula C25H30ClN3. This dye is primarily known for its use in microbiology as a stain, but it has other applications as well.
Crystal Violet can be retained, and analyzed on a Primesep 100 mixed-mode stationary phase column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and a sulfuric acid (H2SO4) buffer. This analysis method can be detected in the UV-Vis regime at 540, 590, and 200 nm.
Methodenparameter
| Column | Primesep 100, 4.6 x 150 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN/H2O – 80/20% |
| Buffer | H3PO4 – 0.2% |
| Flow Rate | 1.0 mL/min |
| Detection | Vis, 590 nm |
| Limit of Detection | 10 ppb |
| Injection Volume | 5 µl |