HPLC Method for Analysis of Cortisone 21-Acetate on Primesep 100 Column
Separation type: Liquid Chromatography Mixed-mode SIELC Technologies
Cortisone 21-acetate is a synthetic corticosteroid used in the treatment of various inflammatory and autoimmune conditions. It is the acetate ester of cortisone, which increases its stability and bioavailability when administered.
Cortisone 21-acetate is a prodrug of cortisone, which is converted to its active form, cortisol, in the liver. Cortisol exerts its effects by binding to glucocorticoid receptors, which modulate the expression of anti-inflammatory proteins and inhibit the expression of pro-inflammatory proteins.
Cortisone 21-Acetate can be retained, separated and analyzed using a Primesep 100 mixed-mode stationary phase column. The analysis employs an isocratic method with a simple mobile phase comprising water, acetonitrile (MeCN), and phosphoric acid as a buffer. This method allows for detection using UV 200 nm.
You can find detailed UV spectra of Cortisone 21-Acetate and information about its various lambda maxima by visiting the following link .
Methodenparameter
| Column | Primesep 100, 4.6 x 150 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN – 50% |
| Buffer | H3PO4 -0.2% |
| Flow Rate | 1.0 mL/min |
| Detection | UV 250 nm |
| Injection Volume | 1 µl |