HPLC Method for Analysis of Cetirizine
Cetirizine is a member of the diphenylmethylpiperazine group of antihistamines and it contains L- and D-stereoisomers. The drug is a major metabolite of hydroxyzine, and a racemic selective H1 receptor antagonist. In general use, it treatments of hay fever, allergy symptoms (crying, itching, sneezing, etc.), and angioedema. Primesep 200, a reverse phase column, contains embedded acidic ionizable groups and can retain Cetirizine. The method is UV compatible and can be used as a general approach for analyzing similar compounds.
Methodenparameter
| Column | Primesep 200, 3.2×100 mm, 5 µm, 100 Å |
|---|---|
| Mobile Phase | Gradient MeCN – 5-50% |
| Buffer | Gradient H3PO4 – 0.05-0.3%, 5 min, 4 min hold |
| Flow Rate | 0.5 mL/min |
| Detection | UV, 270 nm |