HPLC Method for Analysis of Cefuroxime on Primesep 100 Column
Separation type: Liquid Chromatography Mixed-mode
Cefuroxime is a cephalosporin antibiotic used to treat a variety of infections including bronchitis, gonorrhea, and Lyme disease. This antibiotic can be retained and analyzed on a Primesep 100 mixed-mode stationary phase column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and a sulfuric acid (H2SO4) buffer. This analysis method can be detected in the UV regime at 190 and 275 nm.
Methodenparameter
| Column | Primesep 100 , 4.6×150 mm, 5 µm, 100 Å |
|---|---|
| Mobile Phase | MeCN/H2O – 20/80% |
| Buffer | H2SO4 – 0.05% |
| Flow Rate | 1.0 mL/min |
| Detection | UV 190, 275 nm |