HPLC Method for Analysis of Butorphanol
Butorphanol is a synthetic morphinan analgesic. It has narcotic antagonist action and is used to manage severe pain and migraines as a supplement for balanced general anesthesia. Primesep 200, a reverse phase column, contains embedded acidic ionizable groups and can retain Butorphanol. The method is UV compatible and can be used as a general approach for analyzing similar compounds.
Methodenparameter
| Column | Primesep 200, 3.2×100 mm, 5 µm, 100 Å |
|---|---|
| Mobile Phase | Gradient MeCN – 5-50% |
| Buffer | Gradient H3PO4 – 0.05-0.3%, 5 min, 4 min hold |
| Flow Rate | 0.5 mL/min |
| Detection | UV, 270 nm |