HPLC Method for Analysis of Bicine on Primesep 100 Column
Separation type: Liquid Chromatography Mixed-mode
High Performance Liquid Chromatography (HPLC) Method for Analysis of Bicine
Bicine is a common organic buffering agent used in low temperature conditions. It is one of many buffering agents known as “Good’s buffers,” and like many of them, Bicine is zwitterionic. Bicine can be retained and analyzed on a mixed-mode Primesep 100 column with a mobile phase consisting of water, Acetonitrile (MeCN), and Sulfuric acid (H2SO4). This analytical method can be UV detected at 200 nm with high resolution and peak symmetry
Methodenparameter
| Column | Promesep 100, 4.6×150 mm, 100 Å |
|---|---|
| Mobile Phase | MeCN – 20% |
| Buffer | H2SO4 – 0.1% |
| Flow Rate | 1.0 mL/min |
| Detection | UV 200 nm |