HPLC Method for Analysis of Benzoin on Primesep 200 Column
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Benzoin is an organic compound with the chemical formula C14H12O2 . It is primarily used as a precursor to benzil or in powder coating. You can find detailed UV spectra of Benzoin and information about its various lambda maxima by visiting the following link.
Benzoin can be retained and analyzed using the Primesep 200 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a sulfuric acid buffer. Detection is performed using UV.
Methodenparameter
| Column | Primesep 200, 4.6 x 150 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN/H2O – 40/60% |
| Buffer | H2SO4 – 0.1% |
| Flow Rate | 1.0 mL/min |
| Detection | UV 200 nm |