HPLC Method for Analysis of Atorvastatin
High Performance Liquid Chromatography (HPLC) Method for Analysis of Atorvastatin , Lipitor
Atorvastatin is a prescription medication that has C33H35FN2O5as its molecular formula. It is typically used to lower cholesterol, therefore reducing the risk of heart disease. Grapefruit juice can interfere with this medication.
Atorvastatin , Lipitor can be retained and analyzed using the Newcrom R1 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a phosphoric acid buffer. Detection is performed using UV.
Methodenparameter
| Column | Newcrom R1, 3.2 x 100 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN/H2O – 50/50% |
| Buffer | H3PO4 – 0.1% |
| Flow Rate | 0.5 mL/min |
| Detection | UV, 270 nm |