HPLC Method For Analysis Of Alprazolam on Newcrom R1 Column
High Performance Liquid Chromatography (HPLC) Method for Analysis of Alprazolam
Alprazolam, also known as Xanax, is a perscription medication with the C17H13ClN4molecular formula. It is typically used to treat anxiety and panic disorders. It works through enhancing the effects of GABA, which is a calming neurotransmitter in the brain.
Alprazolam can be retained and analyzed using the Newcrom R1 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a phosphoric acid buffer. Detection is performed using UV.
Methodenparameter
| Column | Newcrom R1, 3.2 x 100 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN/H2O – 30/70% |
| Buffer | H3PO4 – 0.2% |
| Flow Rate | 0.5 mL/min |
| Detection | UV, 250 nm |