HPLC Method for Analysis of Allantoin on Primesep 100 Column
High Performance Liquid Chromatography (HPLC) Method for Analysis of Allantoin
Allantoin is found in plants such as wheat sprouts, chamomile, sugar beet, and comfrey. It is used as a moisturizer, which is, as the name suggest, used to moisturize the skin and to treat or prevent skin irritations and dry skin. Primesep 100, a reverse-phase column, contains embedded acidic ionizable groups and can retain Allantoine. The method is LC/MS and UV compatible and can be used as a general approach for analyzing similar compounds.
Methodenparameter
| Column | Primesep 100, 4.6 x 150 mm, 3 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN/H2O – 10/90% |
| Buffer | TFA- 0.1% |
| Flow Rate | 1.0ml/min |
| Detection | UV, 210 nm |