HPLC Method for Analysis of 4-ethoxyaniline on Primesep 100 Column
Separation type: Liquid Chromatography Mixed-mode
High Performance Liquid Chromatography (HPLC) Method for Analysis of 4-ethoxyaniline
4-Ethoxyaniline, also known as p-Phenetidine, is one of three Phenetidine isomers, and is commonly used as an intermediate product in drug and dye synthesis. 4-Ethoxyaniline can be retained and analyzed on a mixed-mode Primesep 100 column with a mobile phase consisting of water, Acetonitrile (MeCN), and Sulfuric acid (H2SO4). This analytical method can be UV detected at 200 nm with high resolution and peak symmetry.
Methodenparameter
| Column | Promesep 100, 4.6×150 mm, 100 Å |
|---|---|
| Mobile Phase | MeCN – 40% |
| Buffer | H2SO4 – 0.2% |
| Flow Rate | 1.0 mL/min |
| Detection | UV 200 nm |